›› 2011, Vol. 23 ›› Issue (3): 186-189.doi: 10.3969/j.issn.1004-616x.2011.03.006

• 论著 • 上一篇    下一篇

Zwint-1及其变异体Zwint-1v在HeLa细胞不同细胞周期中的亚定位

张 驰,李 蕊#,李康生*   

  1. 汕头大学医学院，广东 汕头 515041
  • 收稿日期:2011-03-22 修回日期:2011-04-13 出版日期:2011-05-30 发布日期:2011-05-30
  • 通讯作者: 李康生

The sub-localization of Zwint-1 and Zwint-1v in different cell cycles of HeLa cells

ZHANG Chi，LI Rui #，LI Kang-sheng*   

  1. Shantou University Medical College, Shantou 515041, Guangdong, China
  • Received:2011-03-22 Revised:2011-04-13 Online:2011-05-30 Published:2011-05-30
  • Contact: LI Kang-sheng

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摘要： 探讨Zw10结合因子-1 (zeste white 10 interactor-1，Zwint-1) 及其变异体 (Zwint-1v)在HeLa细胞不同细胞周期中的亚定位。方法：采用胸腺嘧啶核苷双阻断法将HeLa细胞同步化，通过瞬时转染及间接免疫荧光检测，观察Zwint-1及Zwint-1v在HeLa细胞不同细胞周期中的亚定位。结果：在HeLa细胞间期，Zwint-1野生型定位于细胞质，而Zwint-1v定位于细胞核；在分裂期，Zwint-1野生型及Zwint-1v定位于纺锤体与着丝粒上。Zwint-1v定位较野生型更趋近于细胞核。结论： Zwint-1及Zwint-1v在HeLa细胞不同细胞周期的亚定位存在差异。

关键词: Zwint-1, HeLa细胞, 有丝分裂检查站, 亚定位

Abstract: To study the sub-localization of Zwint-1 wild type and variant(Zwint-1v) in different cell cycles of HeLa cells. METHODS：HeLa cells were synchronized using thymidine double-block method. The sub-localization of Zwint-1 wild type and Zwint-1v were identified by fluorescence microscope after transfection and indirect immunofluorescence. RESULTS：In interphase of HeLa cells，Zwint-1 wild type was localized in the cytoplasm， while Zwint-1v were in nucleus. In mitotic phase，Zwint-1 wild type and Zwint-1v were found on the spindle and centromere. Zwint-1v was closer to the nucleus than the wild type. CONCLUSION：Zwint-1 wild type and Zwint-1v during cell cycle have different sub-localization.

Key words: Zwint-1, Zwint-1v, mitotic checkpoint, localization

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